To determine whether knockdown of FOXM1 attenuates the oncogene function of MELK in ESCC cells, two different shRNA oligonucleotides targeting the coding sequence of human FOXM1 (shFOXM1#1 and shFOXM1#2) were stably transduced into MELK-overexpressing KYSE70 and EC109 cells. This evidence concerns the gene MELK and esophageal squamous cell carcinoma.