NFE2L2 and neuroblastoma: To analyze the modulation of the Nrf2-mediated detoxification pathway, we performed RT-qPCR and Western immunoblotting in SH-SY5Y human neuroblastoma cells exposed to compounds 1–6 and CURC at the concentration of 5 μM or to DMF at the concentration of 20 μM for 24 hours (Figure 2).