SH2D3C and encephalomyelitis: To determine the role of the nsP3 MD in the pathogenesis of encephalomyelitis, we infected 2-week-old CD1 mice intracranially with 1,000 PFU of WT SINV and nsP3 MD mutants N24A, G32A, G32E, G32S, Y114A, and TM (Fig. 3).