To assess the effects of MD mutations on replication efficiency in mouse neuronal cells, NSC34 cells were infected (multiplicity of infection [MOI] of 10) with WT SINV and SINV with each of the nsP3 MD single mutations (N24A, G32A, G32E, G32S, and Y114A) or set of mutations (G32E/I113R/Y114N; TM) (Fig. 2). Here, SH2D3C is linked to infection.