Then, we analysed six samples of human AD patients and found that p-S422 Tau and p-Tyr216 GSK-3β were correlated each other, whereas p-Thr180/Tyr182 p38 MAPK were inversely and reciprocally correlated with p-Ser422 and pTyr216 GSK-3β; Patients 2 and 6 showed the features of high concentration Aβ effect on HT22 cells while patients 1, 3 and 4 showed the features of low concentration Aβ effect on HT22 cells; patient 5 did not follow the typical classification (Fig. 7C, sFig. 4A and sFig. 4B). This evidence concerns the gene GSK3B and Alzheimer disease.