NOS2 and melanoma: We further showed that hypergravity (up to 5 g for 24 h) was able to induce an increase in the mRNA expression of eNOS, sGC-β1 subunit, and MRP4/MRP5 in non-metastatic 1F6 melanoma cells, but there were no apparent changes in the mRNA expression of GC-A/GC-B and iNOS compared to 1 g controls [82].