Next, the biomarker quality of ECRG4 and ITIH5 promoter methylation was tested and then compared to a known putative bladder cancer methylation biomarker (NID2) by Methylation Sensitive Restriction Enzyme (MSRE) qPCR at the independent laboratories of Biotype GmbH (Dresden, Germany). This evidence concerns the gene ITIH5 and urinary bladder cancer.