In the present study, we now confirmed a putative prognostic impact as ITIH5 promoter hypermethylation was associated with poor patients’ outcome in the subgroup of advanced tumors (pT > 2) of the TCGA bladder cancer data set, while increased ITIH5 methylation was further shown to correlate with a higher pT status in our second urine cohort. This evidence concerns the gene ITIH5 and urinary bladder cancer.