IFNA1 and infection: To investigate the mRNA of PRRs, IFNs and ISGs in the spleens of H5N6 viruses infected geese, we quantified the mRNA expression of TLR7, TLR3, RIG-I, MDA5, IFN-α, IFN-γ, Mx, and OASL at 12 hours and 3 days post-infection with the two H5N6 viruses.