Figure 9A shows the illustration of different brain regions we used in this study. Analysis of the tumor in the ALTS1C1-bearing mice showed that protein (Figure 9B,C) or gene (Figure 9D) expression levels of MCT4 were higher in tumor necrotic tissues compared with the adjacent tissues. Furthermore, MCT4 expression was further assessed by immunohistochemical staining and was found to be increased in the tumor necrotic areas of mice with GBM (Figure 9E). This evidence concerns the gene SLC16A3 and glioblastoma.