To further confirm the tumor suppressor role of ZNF750 in ESCC, we establish a subcutaneous transplantation tumor model in BALB/c-nu mice using KYSE150 cells with wild-type ZNF750 stable overexpression (KYSE150-ZNF750wt) and the negative control cells (KYSE150-NC). Here, ZNF750 is linked to neoplasm.