Specifically, DOT1L levels were undetectable in normal human CCD841 cells, and increased from the low malignant colon cancer SW480 and SW116 cell lines (Duke's A stage) to the highly malignant colon cancer HCT116, LoVo, DLD-1 and HT-29 cell lines (Duke's B and C stages). This evidence concerns the gene DOT1L and malignant colon neoplasm.