In this study, MZF1 was identified as an independent prognostic factor for poor outcome of NB patients, while HK2 and PGK1 were direct target genes of MZF1. Our gain- and loss-of-function studies indicated that MZF1 promoted aerobic glycolysis, growth, and invasiveness of NB cells, suggesting the oncogenic roles of MZF1 in NB progression. The gene discussed is HK2; the disease is neuroblastoma.