The findings of our study clearly showed that lncRNA Gm12664–001 negatively modulating miR-295-5p and repressing the target of CAV1 expression and lowering TG level in liver suggest that lncRNA Gm12664–001 may play important roles in NAFLD by affecting lipid accumulation, which provides possible mechanisms and warrants further investigations. The gene discussed is CAV1; the disease is metabolic dysfunction-associated steatotic liver disease.