Then, we further investigated the mechanisms of lncRNA Gm12664–001 could regulate lipid droplets and triglyceride (TG) level in mice hepatocytes by negatively modulating miR-295-5p and directly recognizing and depressing the expression of CAV1, which contributes to the pathogenesis of steatosis formation in NAFLD and might provide a potential novel therapeutic target for the treatment of NAFLD. This evidence concerns the gene CAV1 and metabolic dysfunction-associated steatotic liver disease.