The nuclear and cytosolic (mitochondrial) localizations of GLS2 were also revealed in cancer cells transfected with the c-Myc-tagged GLS2 constructs: Western blots of transfected SH-SY5Y and HepG2 cells showed a clear band in both compartments at the expected molecular mass of taggeg-GLS2 (Fig. 2C). This evidence concerns the gene MYC and cancer.