In experiments conducted in triplicate in a human bronchial epithelial cell line, we either augmented or suppressed GSDMB mRNA expression by plasmid transfection or siRNA knockdown, respectively, and obtained expression data for GSDMB, all predicted flow central genes, and target COPD seed genes from RNA-seq profiles of global gene expression (see Methods for details). Here, GSDMB is linked to chronic obstructive pulmonary disease.