To examine H3K27me3 expression in melanoma cell lines in dependence on differentiation status and in the context of proinflammatory stimulation, we analyzed three differentiated (MaMel 15, MaMel 102, MZ 7) and three dedifferentiated (MaMel 65, MaMel 54a, MaMel 85) human melanoma cell lines, which were stimulated with IFNy or TNFα for 72 h. This evidence concerns the gene TNF and melanoma.