GSTM1 and hemoglobinopathy: Alkaline haemoglobin electrophoresis method was carried out to detect the coinheritance of hemoglobinopathy mainly the sickle cell anaemia, heterozygous B-thalassemia, Hb H or Hb Barts, using SAS-MX Alkaline Hb-10 kit (Helena Bioscience Europe), then the types and percent of Hb were measured by densitometry method.