Moreover, the carcinogenesis and tumor metastasis-related signaling pathways including the upregulation of O-GlcNAcylation (OGA), Ephrin A4 (EFNA4), special AT-rich sequence-binding protein 1 (SATB1), and long non-coding RNA colorectal neoplasia differentially expressed (CRNDE), and downregulation of JUNB, SMARCA4 signaling pathways were observed in TIM-3+, compared with TIM-3− T cell (Figure 4F). This evidence concerns the gene EFNA4 and neoplasm.