CD40LG and infection: A positive IgM Western blot result (2 out of 3 antibody targets detected: P24, P39, P41) can be used to diagnose LD cases within 30 days of infection and a positive IgG Western blot result (5 out of 10 antibody targets detected: P18, P21, P28, P30, P39, P41, P45, P58, P66, P93) is used in cases of potentially longer infection [13–15].