The western blot and the band intensity analysis hinted that over-expression of TUSC8 down-regulated the expression of mesenchymal related markers (ZEB1, TWIST, SNAI1 and Vimentin) in breast cancer cell lines SK-BR-3 and MDA-MB-435 (p < 0.05, p < 0.01 respectively) (Figure 3A). This evidence concerns the gene VIM and breast cancer.