Conversely, knock-down of TUSC8 up-regulated the expression of mesenchymal related markers (ZEB1, TWIST, SNAI1 and Vimentin), and down-regulated the expression of epithelial related marker (E-cadherin) in breast cancer cell lines MCF-7 and HCC1937 (p < 0.05, p < 0.01 respectively) (Figure 3B). This evidence concerns the gene TWIST1 and breast cancer.