Furthermore, the IHC staining of EMT related markers in tumor tissues indicated that the expression of mesenchymal markers (including ZEB1, TWIST, SNAI1 and Vimentin) were significantly up-regulated and the epithelial marker E-cadherin expression was down-regulated in TUSC8 knockdown group compared with the control group (p < 0.01, p < 0.001 respectively) (Figure 4E). Here, TWIST1 is linked to neoplasm.