To determine whether Isorhamnetin can induce apoptotic death in androgen-independent DU145 and PC3 cells, next, both cancer cells were exposed to Isorhamnetin (5, 10, and 20 μM) for 48 h and then the apoptosis was examined by flow cytometry in treated cells double-stained with Annexin V and PI. Here, ANXA5 is linked to cancer.