TENT2 and neoplasm: To examine the dependence of gld-2(−/−) gld-1(−/−); puf-8(−/−) and gld-2(+/−) gld-1(+/−); puf-8(−/−) germline tumor formation on MPK-1 activity, we performed lip-1(RNAi) (MPK-1 activation) or mpk-1(RNAi) (MPK-1 inhibition) on synchronized L1 larvae at a moderate temperature (23°C).