Exogenous transfected miR-27a led to a decreased level of EGFR and inhibition of miR-27a led to EGFR increased in cSCC cells, indicating that miR-27a suppressed the expression of EGFR by binding to 3′UTR region, which is consistent with the tumor suppressive role of miR-27a in cSCC. The gene discussed is EGFR; the disease is skin squamous cell carcinoma.