In the present study, changes in sTILs, PD-L1 expression and vascular normalization were examined in BC patients before and after NAC, the effect of NAC on the TME of BC was investigated, and its correlation with the post-NAC pCR rate and disease-free survival (DFS) was analyzed to find appropriate predictive and prognostic biomarkers for treatment of BC. This evidence concerns the gene CD274 and breast cancer.