This dissimilarity between the cancer cells and the time course data was also observed for ZEB2, which showed the largest absolute change in mean between I2 and M. Consistent with the moderate increase of M score from E to I0, expression of ZEB1, TWIST1, and TWIST2 was not significantly different between I0 and E clusters (Welch's t-test, p = 2.66 × 10−1, 1.43 × 10−1, and 4.20 × 10−1 respectively). This evidence concerns the gene TWIST2 and cancer.