For glioma diagnosis, the AUC obtained from the ROC curve was 0.906 (0.850–0.962) for serum sPD-L1, which was higher than that for the NLR [0.628 (0.535–0.720)], WBCs [0.667 (0.578–0.755)], neutrophils [0.672 (0.582–0.761)], and monocytes [0.657 (0.567–0.748)]. The gene discussed is SPDL1; the disease is central nervous system cancer.