CD40 and infection: The CpG-recoded E+102CpG and E+176CpG ZIKV variants caused impaired infection in moDCs as compared to the WT and E+32CpG variants (Figure 5); however, in accordance with previous studies with wild-type ZIKV (64–66), activation of moDCs was not induced by any ZIKV variant as represented by similar expression of HLA-DR, CD40, CD80, and CD86 (Figures 5D–G).