To investigate the impact of the TG2 inhibitors on autophagy, THP-1 mRFP-GFP-LC3B cells were infected with Mtb H37Rv Ds-Red Cherry and then treated with rapamycin, cystamine, and cysteamine immediately after infection, and AIPD was measured at 24 h later (Figure 3). Here, MAP1LC3B is linked to infection.