We applied TRAS to investigate the axon projection patterns of retinorecipient cells and identified potential connectivity changes in zebrafish carrying a mutation in down syndrome cell adhesion molecule-like 1 (dscaml1), a causal gene for autism spectrum disorder and human cortical abnormalities (Fuerst et al., 2009; Iossifov et al., 2014; Karaca et al., 2015; Galicia et al., 2018; Ma et al., 2020). Here, DSCAML1 is linked to autism spectrum disorder.