Epithelial (E‐cadherin) and mesenchymal (N‐cadherin and Vimentin) biomarkers as well as cell morphological changes are major EMT indicators.32 TGF‐β1 is a key player in cancer microenvironment modification and promotion of EMT, which could enhance breast cancer cell tumorigenesis, migration, and invasion.33, 34 In our study, we demonstrated that compared with control cells, TGFβ1‐treated MDA‐MB‐231 cells exhibited an elongated fibroblast‐like shape. This evidence concerns the gene VIM and breast carcinoma.