Pertuzumab and trastuzumab alone were each more effective in the HER2-negative primary tumor cell group than in the HER2+ cell line group, consistent with previous studies using breast cancer cell lines (Agus 2002; Junttila et al. 2009), Pertuzumab inhibited NRG1b-initiated HER2 signaling by an average of 62% (range 47–83%) in HER2+ cell lines and an average of 73% (range 68–75%) in HER2-patient cell samples (Fig. 3). Here, ERBB2 is linked to breast cancer.