To further probe whether the nuclear targeting of UL2 influences the viral replication of HSV-1, stocks of WT HSV-1 (vUL2) and recombinant viruses (vUL2Del, vUL2Mu and vUL2Rev) were prepared and their titers were determined, then the plaque formation and viral propagation property of each recombinant virus were tested at an multiplicity of infection (MOI) of 1. The gene discussed is RPL8; the disease is infection.