We measured PGE2 upon ACSL3 knockdown with two different shRNAs, and found that the loss of ACSL3 caused either a mild decrease (H596) or had no effect (H838) in PGE2 production in the ACSL3-independent cancer cell lines, while it highly suppressed PGE2 production in the ACSL3-dependent cell lines H125 and HCC95 (Supplementary Fig. 1F). This evidence concerns the gene ACSL3 and cancer.