Of note, some cancer-associated gene mutations identified in a single metastatic lesion, such as those in BRCA2 (c.1318delC, unique to L1), APC (c.6053–6071delCAGTTTGTTTCTCAAGAAA, unique to L1), FBXW7 (c.365C > A, unique to L2), and PTEN (c.650T > G, unique to N2), were also detectable in plasma cfDNA. Here, PTEN is linked to cancer.