To investigate whether inhibition of cancer cell invasion by TSWU-BR4 results in a dysregulation of the lipid raft compartmentalization of phosphatidylinositol 3-kinase (PI3K)−protein kinase B (Akt) signaling-regulated molecules, a sucrose density-based membrane flotation experiments after 1% Brij 98 solubilization of TSWU-BR4-treated cells was performed to isolate detergent-resistant membranes (DRMs), which are biochemically defined as the lipid rafts [2]. The gene discussed is AKT1; the disease is cancer.