Immunofluorescence analysis showed a significant reduction of E-cadherin expression when HT-29 cells were cultured in CRLM scaffolds compared to HL and CRC samples (p-value < 0.001 and p-value < 0.0001, respectively) (Figure 4A), whereas they exhibited a significantly increased expression of Vimentin only when cultured in CRLM scaffolds compared to CRC and both healthy scaffolds (p-value < 0.001 vs. CRC; p-value < 0.001 vs. HL and p-value = 0.001 vs. HC) (Figure 4B). Here, VIM is linked to colorectal carcinoma.