EGFR and non-small cell lung carcinoma: Several NSCLC cell lines with/without EGFR mutation were fixed using four different methanol-based fixative solvents, 55% methanol, and 55% methanol including 0.4% formaldehyde, PreservCyt Solution for ThinPrep (TP) (for non-gynecologic purposes), or BD CytoRich red preservative (CR).