PCNA and cancer: To confirm that long-term effect of deficiency of BRG1 activity on gene transcription is mediated by BRG1 gene targets which drive cancer cell proliferation (e.g., CDK4, PCNA, CCNB), we checked their transcription rate and chromatin features which are representative of BRG1/EP300 proximate effects (H3 density and nucleosome acetylation), and also looked for cell cycle-related chromatin remodeling (RB1) after short-term MCF7 cell treatment with inhibitors (Figure 4B,C).