Here, A2058 (Figure 1A–C) and A375 (Figure S4A–C) melanoma cells were exposed to 0.2 μg/mL recombinant WNT5A (rWNT5A) protein for different time periods (starting at 15 minutes to a maximum of 24 hr) to observe any changes in total expression and Ser-159/163 phosphorylation levels of MARCKS. Here, WNT5A is linked to melanoma.