In association with the above cell death increase at 48 h (Fig. 6), analysis of kidneys from FA-AKI mice revealed the development of critical features of ferroptosis, such as increased lipid peroxidation as assessed by increased 4-hydroxynonenal (4-HNE) staining by immunohistochemistry (Fig. 8A) and IL-33 proteolytic processing to its proinflammatory form as evaluated by Western blot (Fig. 8B) (Supplementary Fig. S4). Here, IL33 is linked to acute kidney injury.