Furthermore, an in vivo tumour formation experiment by subcutaneously injecting CD133+ CSCs without any transfection (Blank group), CD133+ CSCs transfected with pcDNA3.0-PinX1 (PinX1 group) and CD133+ CSCs transfected with pcDNA3.0-PinX1 and miR-200b inhibitor (PinX1 + miR-200b inhibitor group) into nude mice were conducted to confirm the effects concerning PinX1 and miR-200b axis (Fig. 6). The gene discussed is PINX1; the disease is neoplasm.