From in vitro studies, after osthole intervention (20, 40, and 80 mol/L) on melanoma A375 cells, the mRNA level of Bcl-2 declined, same as the expression of p-NF-κB and Bcl-2 protein level in a dose-dependent manner, the mRNA level of Bax and the expression of IκB-α increased. This evidence concerns the gene NFKB1 and melanoma.