Results also showed the following to be remarkably regulated by Notch mutation in GC: biological processes such as receptor activity, calcium ion binding and enzyme binding; cellular components such as Golgi membrane, endoplasmic reticulum membrane, and receptor complex; molecular functions such as Notch signaling pathway and transcription initiation from RNA polymerase II promoter; KEGG disease terms such as cancers, cancers of the digestive system and musculoskeletal diseases; KEGG pathways such as cell cycle, Notch signaling pathways and TGF-β signaling pathway. This evidence concerns the gene TGFB1 and musculoskeletal system disorder.