Indeed for each approved drug targeting the programmed death receptor (PD)1/programed death ligand 1 (PD-L1) axis, namely, Atezolizumab, Pembrolizumab, Nivolumab and Durvalumab, a different screening assay is suggested by the Food and Drug Administration (FDA) and European Medical Agency (EMA) that not only use different antibodies (Abs) for staining PD-L1 (SP142, 22c3, 28-8 and SP263, respectively), but also different cut off values, which take into consideration membranous/cytoplasmic staining of tumor and/or immune cells. This evidence concerns the gene CD274 and neoplasm.