To evaluate the effect of NVP-BEZ235 on cell proliferation in thyroid cancer cells with different TP53 status, we cultured the cells with 500 nM NVP-BEZ235 or vehicle control, and using MTT assays to determine the cell viability on different time points (0, 12, 24, 36, 48 and 72 h). The gene discussed is TP53; the disease is thyroid gland carcinoma.