In the present study, we verified that lncRNA RUNX1-IT1 was mainly located in the cytoplasm and the expression of lncRNA RUNX1-IT1 was reduced in HCC samples and HCC cell lines, and the downregulation of RUNX1-IT1 in tumours was validated to be a predictor of worse survival in HCC patients. Here, RUNX1 is linked to neoplasm.