TDP2 and infection: We tested virus protein production in the absence of TDP2 both qualitatively by Western blot analysis and quantitatively using a Renilla luciferase-expressing poliovirus reporter [39], termed PV-PPP, which is depicted in Figure 8B. We infected the WT and KO TDP2 hRPE-1 cells with either WT poliovirus or the luciferase reporter virus and harvested infected cells at 2 h intervals throughout the 8 h time course of infection.