In order to investigate the relationship between PGE2 and cytokine production, IL-1β, IL-6, and TNF-α were measured by qRT-PCR, whereas IL-6, TNF-α,IL-12 and IL-23 were measured by ELISA, and these were obtained from DCs cultures with or without the infection of either M. bovis or BCG after PGE2 was inhibited by the COX inhibitor. The gene discussed is IL6; the disease is infection.