The findings of function annotation revealed a number of unreported signaling pathways in gliomas which PDIs may be involved in and included P53 signaling pathway, cell cycle, tumor necrosis factor (TNF) mediated signaling pathway, regulation of MAPK cascade, JAK-STAT signaling pathway, positive regulation of NF kappa B transcription factor activity, regulation of tyrosine phosphorylation of STAT3 protein, vascular endothelial growth factor receptor signaling pathway, DNA replication, and mismatch repair. The gene discussed is TNF; the disease is central nervous system cancer.