The network was used to investigate the role of CPS1, which codes for a protein that catalyzes the first step of the urea cycle, and CAD, which codes for a trifunctional protein catalyzing the first three steps of de novo pyrimidine biosynthesis pathway, in normal metabolism, and in turn, metabolic reprogramming in HCC.7,8,22–25. This evidence concerns the gene CAD and hepatocellular carcinoma.