This study aimed to identify the evidence for CPS1 and carbamoyl phosphate synthetase/aspartate transcarbamoylase/dihydroorotase (CAD) dysregulation in HCC based on existing literature and explore their metabolic impact through the reconstruction of a network that integrates information from the KEGG, HPA and HMDB databases. This evidence concerns the gene CAD and hepatocellular carcinoma.