We observed that NNT-AS1 was overexpressed in CCA tissues and cell lines and took promotion roles in CCA growth presented as elevating cell proliferation, EMT and PI3K/AKT and ERK1/2 pathways via down-regulating miR-203 in CCLP1 and TFK1 cells. The gene discussed is MAPK3; the disease is cholangiocarcinoma.